|
KMID : 0360220190600100975
|
|
Journal of the Korean Ophthalmological Society
2019 Volume.60 No. 10 p.975 ~ p.981
|
|
|
Role of Hydrogen Sulfide in the Survival of Fibroblasts and Fibroblast-mediated Contraction of Collagen Gel
|
|
Park Hyeon-Jin
Kim Jae-Woo
|
|
|
Abstract
|
|
|
Purpose: To investigate the role of hydrogen sulfide in the survival and collagen gel contraction of cultured human Tenon’s capsule fibroblasts (HTCFs).
Methods: Primarily cultured HTCFs were exposed to 0, 100, 200, or 300 μM hydrogen sulfide (sodium hydrogen sulfide, NaHS) for 2 days. Cellular survival was assessed by MTT (3-[4, 5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide) assay. Degree of apoptosis was assessed with flow cytometry using annexin-V/propidium iodide double staining. To evaluate the effect of NaHS on cellular transdifferentiation, HTCFs were stimulated with 5 ng/mL TGF-β1 and the level of expression of α-smooth muscle actin (SMA) mRNA was assessed using reverse-transcription polymerase chain reaction. The cells were embedded in collagen gel, and the amount of gel contraction was measured.
Results: NaHS at 300 μM reduced HTCF survival (p = 0.013); NaHS at both 200 and 300 μM increased apoptosis in a dose-dependent manner (p = 0.013 and p = 0.016). TGF-β1 increased the expression of α-SMA mRNA (p = 0.041); co-treatment with 100 μM NaHS decreased TGF-β1-induced α-SMA mRNA expression (p = 0.039) and inhibited collagen gel contraction.
Conclusions: NaHS at high concentration reduced cellular survival and increased HTCF apoptosis. NaHS decreased TGF-β 1-induced increases in α-SMA mRNA expression and collagen gel contraction. Thus, hydrogen sulfide may suppress scar formation by inhibiting HTCF transdifferentiation and contraction of collagen gels.
|
|
|
KEYWORD
|
|
|
Apoptosis, Hydrogen sulfide, Survival, Transdifferentiation, Tenon capsule fibroblasts
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
  
|
|